Bodycare, on Cambridge Street in Harrogate, will finally be closing its doors after being one of the 56 stores to survive the first round of closures earlier this month.
The high street beauty chain collapsed into administration last month and closed 30 of its stores.
A shortage of stock and the cost of running stores meant it was no longer viable to keep its 115 stores open, administrators said at the time.
The latest round of closures, including that of the Harrogate store, will result in 444 redundancies, according to administrators.
Bodycare specialised in selling fragrances, toiletries, cosmetics and skincare products and was founded on a Lancashire market stall more than half a century ago. It employed about 1,500 people.

Pateley Bridge to host first New Year's Day cyclo-cross event
Harrogate MP welcomes government U-turn on family farm inheritance tax
Santa Paws: Festive photo competition winners revealed
DalesBus reveals timetable over Christmas period
Festive tractor run raises £45,000 for charity
CCTV appeal after Ripon shop theft
Yorkshire Vet Julian Norton meets fans in Harrogate
Santa delivers gifts to Harrogate children on all-electric sleigh
Rudy's Pizza set to open new Harrogate restaurant
75-year-old Ripon cyclist completes epic birthday challenge
Miriam Margolyes to visit Harrogate as part of upcoming tour
Towns must see benefits of any tourist tax, warns Harrogate MP
Police appeal for wanted man who could be in Ripon
Harrogate school 'takes Law into its own hands' with rare A Level course
CCTV appeal after motorbike stolen in Harrogate
Specsavers Harrogate hosts free after-hours clinic for homeless residents
Man charged following Pateley Bridge hit-and-run
Banyan in Harrogate set to close
Knaresborough boys 'devastated' after beloved cat goes missing
Knaresborough tug-of-war tradition to return on Boxing Day


